Aaron Ramsey has been forced to pull out of Wales' Nations League games with the Republic of Ireland and Finland this month.
The Juventus midfielder has withdrawn from the squad due to a thigh injury he picked up in their Champions League win over Ferencvaros last night.
"Aaron Ramsey, who was substituted off during last night’s match, was subjected to examinations at J | Medical this morning," the Italian club announced.
"It revealed a low-grade injury to the rectus femoris muscle of the right thigh. In 10 days it will be re-evaluated again."
Injuries have hampered the former Arsenal player's international career, Ramsey's made only two starts for Wales in the last two years.
Rob Page will take charge of Wales for the games against Ireland and Finland as well as their friendly against the USA, after their manager Ryan Giggs was arrested.
Giggs had previously named the squad for the upcoming matches.
Wales squad
Goalkeepers: Daniel Ward, Owain Fon Williams, Tom King
Defenders: Chris Gunter, Ben Davies, Connor Roberts, Ethan Ampadu, Chris Mepham, Tom Lockyer, Joe Rodon, Neco Williams, James Lawrence, Ben Cabango, Rhys Norrington-Davies
Midfielders: Jonathan Williams, Harry Wilson, David Brooks, Daniel James, Matthew Smith, Joe Morrell, Dylan Levitt, Brennan Johnson, Josh Sheehan
Forwards: Gareth Bale, Tom Lawrence, Kiefer Moore, Tyler Roberts, Rabbi Matondo
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